Journal: Oncology Letters

Article Title: Antitumor activity of nimotuzumab in combination with cisplatin in lung cancer cell line A549 in vitro

doi: 10.3892/ol.2018.7923

Figure Lengend Snippet: Cyclin D1 expression in A549 cells in groups A-E following treatment for 48 h. Lanes: A, nimotuzumab; B, cisplatin; C, nimotuzumab followed by cisplatin; D, nimotuzumab and cisplatin simultaneously; and E, untreated control.

Article Snippet: Cell culture The A549 human lung adenocarcinoma epithelial cell line (supplied by the Central Laboratory of Qinhuangdao No. 1 People's Hospital) was cultured in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Zhejiang Tianhang Biotechnology Co., Ltd., Huzhou, China), 1 U/ml penicillin and 1 mg/ml streptomycin at 37°C, with 5% CO 2 and 95% humidity.

Techniques: Expressing, Control

Journal: Frontiers in Molecular Biosciences

Article Title: Comparison of two immunotoxins against DLL3 receptor; as an inhibitor for small cell lung cancer

doi: 10.3389/fmolb.2025.1506768

Figure Lengend Snippet: (A, B) The cell viability percentage of Rova-GrB and Rova-Typh on A549 and HUVEC cell lines after 24 h of incubation (The control group is without Rova-GrB and Rova-Typh). The significance of the results (p-Value) between the control group and other groups has been indicated as follows: *P < 0.05; **P < 0.01; **P < 0.001.

Article Snippet: The human A549 lung cancer cell lines and the normal HUVEC, Human umbilical vein endothelial cell line, were purchased from the Pasteur Institute and Iranian Biological Resource Center (IBRC), respectively.

Techniques: Incubation, Control

Journal: Nano Convergence

Article Title: Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip

doi: 10.1186/s40580-021-00285-4

Figure Lengend Snippet: Effect of ECM on the differentiation of kidney organoids in a static culture condition. A Fluorescent images of immunostained kidney organoids cultured with various conditions of ECMs for 6 days. The cells were immunostained by NPHS1 (podocyte, red), PECAM1 (vascular endothelial cell, green), LTL (proximal tubules, white), and DAPI (nuclei, blue). B Quantitative analysis of kidney antibody-specific markers from kidney organoids (* indicates p <0.05 for experimental group vs. control group). Scale bars are 100 μm

Article Snippet: Primary antibodies against the following proteins were used to characterize various kidney-related cell types: podocytes (anti-NPHS1, R&D AF4269, 1:500), proximal tubular cells (anti-LTL, Vector Labs FL‐1321, 1:500), and ECs (anti-PECAM1, Abcam ab9498, 1:200).

Techniques: Cell Culture

Journal: Nano Convergence

Article Title: Effect of biochemical and biomechanical factors on vascularization of kidney organoid-on-a-chip

doi: 10.1186/s40580-021-00285-4

Figure Lengend Snippet: Effect of the shear stress on the differentiation of kidney organoids in a kidney organoid-on-a-chip. A Fluorescent images showing hPSCs-derived kidney organoid-related cell differentiation under a shear stress condition. Kidney organoids were cultured within kidney organoid-on-a-chip for 6 days. After culturing for 6 days, the cells were stained for NPHS1 (podocyte, red), PECAM1 (vascular endothelial cell, green), LTL (proximal tubules, white), and DAPI (nuclei, blue) to identify the kidney-related cells, respectively. B Quantitative analysis of the kidney-related markers from kidney organoids. (* indicates p < 0.05 for LTL expression of Matrigel-coated group vs. Matrigel/VEGF-coated group, *** indicates p < 0.001 for PECAM expression of Matrigel-coated group vs. Matrigel/VEGF-coated group). Scale bars are 100 μm

Article Snippet: Primary antibodies against the following proteins were used to characterize various kidney-related cell types: podocytes (anti-NPHS1, R&D AF4269, 1:500), proximal tubular cells (anti-LTL, Vector Labs FL‐1321, 1:500), and ECs (anti-PECAM1, Abcam ab9498, 1:200).

Techniques: Derivative Assay, Cell Differentiation, Cell Culture, Staining, Expressing

Journal: Cancer Control : Journal of the Moffitt Cancer Center

Article Title: Over-Expression of ARID3B Suppresses Tumor Progression and Predicts Better Prognosis in Patients With Gastric Cancer

doi: 10.1177/10732748231169403

Figure Lengend Snippet: ARID3B suppresses gastric cancer cell migration and metastasis in vitro. (A) Wound healing assay showed that the relative healing of wounded areas in the over-expression of ARID3B groups were reduced compared with those in the control groups in 48 h. The percentage of wound area closure was analyzed by Image J. (B) The relative healing of wounded areas in the ARID3B knockdown groups were increased compared with those in the control groups in 48 h. The percentage of wound area closure was analyzed by Image J. (C) Compared with the control cells, ARID3B over-expression SGC-7901 cells expressed lower level of N-cadherin, snail, twist and vimentin, while ARID3B knockdown AGS cells expressed higher at protein level by WB. Original magnification:×10, scale bar 500 μm. Data are presented as the mean ± SD of 3 independent experiments. * P < .05, ** P < .01, *** P < .001, **** P < .0001.

Article Snippet: Next, the membranes were fully incubated with antibodies specific for ARID3B (abcam, ab227870, 1:1000), N-cadherin (abcam, ab98952, 1:500), snail (abcam, ab31787, 1:500), twist (abcam, ab175430, 1:500), vimentin (abcam, ab92547, 1:1000) and GAPDH (abcam, ab8245, 1:5000), overnight at 4°C.

Techniques: Migration, In Vitro, Wound Healing Assay, Over Expression